S. J. Higgins, Protein Expression: A Practical Approach
ISBN: 0199636249 | edition 1999 | PDF | 303 pages | 17 mb
Part of a mini-series on the synthesis and processing of proteins, this volume details the expression of cloned DNA and RNA templates in all of the major in vivo and in vitro systems. The in vivo systems covered are cultured mammalian cells, the yeasts Sacchoromyces cerevisiae and Pichia pastoris, baculovirus, Xenopus oocytes, and prokaryotic cells. It also covers cell-free systems of both eukaryotes and prokaryotes, including prokaryotic systems that offer coupled transcription-translation. The volume concludes with a chapter on monitoring protein expression.